This three-year study at the Sevilleta LTER was designed to monitor net primary production (NPP) across two distinct ecosystems: pinon/juniper woodland (P) and juniper savannah woodland (J). Net primary production (NPP) is a fundamental ecological variable that measures rates of carbon consumption and fixation. Estimates of NPP are important in understanding energy flow at a community level as well as spatial and temporal responses of the community to a wide range of ecological processes. While measures of both below- and above-ground biomass are important in estimating NPP, this study focused on estimating above-ground biomass production (ANPP).
To measure ANPP (i.e., the change in plant biomass, represented by stems, flowers, fruit and foliage, over time), the vegetation variables in this dataset, including species composition and the cover and height of individuals, were sampled twice yearly (spring and fall) at permanent 1m x 1m plots. The data from these plots was used to build regressions correlating biomass and volume via weights of select harvested species obtained in SEV157, "Net Primary Productivity (NPP) Weight Data." In addition, volumetric measurements were obtained from permanent plots to build regressions correlating biomass and volume.
Spring measurements were taken in April or May when shrubs and spring annuals reached peak biomass. Fall measurements were taken in either September or October when summer annuals reached peak biomass but prior to killing frosts. Winter measurements were taken in February before the onset of spring growth.
Collecting the Data:
Vegetation data is collected on a palm top computer. Excel spreadsheets are used for data entry and file names should begin with the overall study (npp), followed by the date (mm.dd.yy) and the initials of the recorder (.abc). Finally, the site abbreviation should be added (i.e., c, g, b, p). The final format should be as follows: npp.mm.dd.yy.abcg.xls. File names should be in lowercase.
A 1-m2 PVC-frame is placed over the fiberglass stakes that mark the diagonal corners of each quadrat. When measuring cover it is important to stay centered over the vegetation in the quadrat to prevent errors caused by angle of view (parallax). Each PVC-frame is divided into 100 squares with nylon string. The dimensions of each square are 10cm x 10cm and represent 1 percent of the total area.
The cover (area) and height of each individual live (green) vegetative unit that falls within the one square meter quadrat is measured. A vegetative unit consists of an individual size class (as defined by a unique cover and height) of a particular species within a quadrat. Cover is quantified by counting the number of 10cm x 10cm squares filled by each vegetative unit.
Niners and plexidecs are additional tools that help accurately determine the cover a vegetative unit. A niner is a small, hand-held PVC frame that can be used to measure canopies. Like the larger PVC frame it is divided into 10cm x 10cm squares, each square representing 1% of the total cover. However, there are only nine squares within the frame, hence the name “niner.” A plexidec can help determine the cover of vegetative units with covers less than 1%. Plexidecs are clear plastic squares that are held above vegetation. Each plexidec represents a cover of 0.5% and has smaller dimensions etched onto the surface that correspond to 0.01%, 0.05%, 0.1%, and 0.25% cover.
It is extremely important that cover and height measurements remain consistent over time to ensure that regressions based on this data remain valid. Field crew members should calibrate with each other to ensure that observer bias does not influence data collection.
Grasses-To determine the cover of a grass clump, envision a perimeter around the central mass or densest portion of the plant, excluding individual long leaves, wispy ends, or more open upper regions of the plant. Live foliage is frequently mixed with dead foliage in grass clumps and this must be kept in mind during measurement as our goal is to measure only plant biomass for the current season. In general, recently dead foliage is yellow and dead foliage is gray. Within reason, try to include only yellow or green portions of the plant in cover measurement while excluding portions of the plant that are gray. This is particularly important for measurements made in the winter when there is little or no green foliage present. In winter, sometimes measurements will be based mainly on yellow foliage. Stoloniferous stems of grasses that are not rooted should be ignored. If a stem is rooted it should be recorded as a separate observation from the parent plant.
Forbs-The cover of forbs is measured as the perimeter of the densest portion of the plant. If the forb is an annual it is acceptable to include the inflorescence in this measurement. If the forb is a perennial, do not include the inflorescence as part of the cover measurement. Measure all foliage that was produced during the current season, including any recently dead (yellow) foliage. Avoid measuring gray foliage that died in a previous season.
Cacti-For cacti that consist of a series of pads or jointed stems (Opuntia phaecantha, Opuntia imbricata) measure the length and width of each pad to the nearest cm instead of cover and height. Cacti that occur as a dense ball/clump of stems (Opuntia leptocaulis) are measured using the same protocol as shrubs. Pincushion or hedgehog cacti (Escobaria vivipara, Schlerocactus intertextus, Echinocereus fendleri) that occur as single (or clustered) cylindrical stems are measured as a single cover.
Yuccas-Make separate observations for the leaves and caudex (thick basal stem). Break the observations into sections of leaves that are approximately the same height and record the cover as the perimeter around this group of leaf blades. The caudex is measured as a single cover. The thick leaves of yuccas make it difficult to make a cover measurement by centering yourself over the caudex of the plant. The cover of the caudex may be estimated by holding a niner next to it or using a tape measure to measure to approximate the area.
Height is recorded as a whole number in centimeters. All heights are vertical heights but they are not necessarily perpendicular to the ground if the ground is sloping.
Annual grasses and all forbs-Measure the height from the base of the plant to the top of the inflorescence (if present). Otherwise, measure to the top of the green foliage.
Perennial grasses-Measure the height from the base of the plant to the top of the live green foliage. Do not include the inflorescence in the height measurement. The presence of live green foliage may be difficult to see in the winter. Check carefully at the base of the plant for the presence of green foliage. If none is found it may be necessary to pull the leaf sheaths off of several plants outside the quadrat. From this you may be able to make some observations about where green foliage is likely to occur.
Perennial shrub and sub-shrubs-Measure the height from the base of the green foliage to the top of the green foliage, ignoring all bare stems. Do not measure to the ground unless the foliage reaches the ground.
Plants rooted outside but hanging into a quadrat-Do not measure the height from the ground. Measure only the height of the portion of the plant that is within the quadrat.
Foliage canopy cover:
Cover and height are recorded for all separate vegetative units that fall within an infinite vertical column that is defined by the inside edge of the PVC-frame. A vegetative unit consists of an individual species with a unique cover and height. This includes vegetation that is rooted outside of the frame but has foliage that extends into the vertical column defined by the PVC-frame.
As mentioned above, cover is quantified by counting the number or fraction of 10 cm x 10 cm squares intercepted by each vegetative unit. It is possible to obtain a total percent cover greater than 100 for a quadrat because vegetative units often overlap (especially in shrubs and succulents). For perennial plants, cover is based only on the vegetative portion of the plant (stem and leaf). For annual plants, cover is based on both vegetative and reproductive (inflorescence) portions of the plant.
If the cover of a vegetative unit is less than 1, the increments used are as follows: 0.01, 0.05, 0.1, 0.25, 0.5, and 0.75. If cover is between 1 and 5, increments of 0.5 are used and, if greater than 5, increments of 1 are used. Finally, if the cover is greater than 15, the total canopy cover is divided into smaller units and the cover and heights of each observation measured separately. This reduces the size of harvest samples.
January 7, 2008 KRW Data from the P and J sites from 1999 to 2002 were extracted from the ongoing npp database and put in its own table in navicat. Palmtop/pj_npp. NPP data from 1999-2001 was QAQC'd in MySQL. I checked for duplicates and missing quads. These most often happened when a recorder mislabeled a particular quad. I also checked every plant code against the USDA Plants database online at http://plants.usda.gov/. All plant codes that have had nomenclature changes were updated. All previously unknown plants that have since been identified were also updated. All unknown plants that will never be identified were left in the database. All types were corrected. A list of codes not in the USDA list are that are still in the data are as follows NONE = no plants in quad, and UKFO57 = unknowns that will never be identified, UKFO80 = unknown that has not yet been identified. A list of the updates and the reason for the change are in the table below along with comments where identifications were questionable.
Employee History:Mike Friggens: 1999 to September 2001, Karen Wetherill: February 7, 2000 to August 2009, Terri Koontz: February 2000 to August 2003 and August 2006 to August 2010, Shana Penington: February 2000 to August 2000, Heather Simpson: August 2000 to August 2002, Chris Roberts: September 2001 to August 2002.
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