The Monsoon Rainfall Manipulation Experiment (MRME) is to understand changes in ecosystem structure and function of a semiarid grassland caused by increased precipitation variability, which alters the pulses of soil moisture that drive primary productivity, community composition, and ecosystem functioning. The overarching hypothesis being tested is that changes in event size and variability will alter grassland productivity, ecosystem processes, and plant community dynamics. In particular, we predict that many small events will increase soil CO2 effluxes by stimulating microbial processes but not plant growth, whereas a small number of large events will increase aboveground net primary production (ANPP) and soil respiration by providing sufficient deep soil moisture to sustain plant growth for longer periods of time during the summer monsoon.
To measure ANPP (i.e., the change in plant biomass, represented by stems, flowers, fruit and foliage, over time), the vegetation variables in this dataset, including species composition and the cover and height of individuals, are sampled twice yearly (spring and fall) at permanent 1m x 1m plots. The data from these plots is used to build regressions correlating biomass and volume via weights of select harvested species obtained in SEV157, "Net Primary Productivity (NPP) Weight Data." This biomass data is included in SEV206, "Seasonal Biomass and Seasonal and Annual NPP for the Monsoon (MRME) Study."
The Monsoon Rainfall Manipulation Experiment (MRME) contains three ambient precipitation plots and five replicates of the following treatments: 1.) ambient plus a weekly addition of 5 mm rainfall; and 2.) ambient plus a monthly addition of 20 mm rainfall. Rainfall is added during the monsoon season (July-Sept) by an overhead (7 m) system fitted with sprinkler heads that deliver rainfall quality droplets. At the end of the summer, each treatment has received the same total amount of added precipitation delivered in different sized events.
Each plot (9m x 14 m) includes subplots (2m x 2m) that receive 50 kg N ha-1 y-1. Measurements are taken of: 1.) seasonal (July, August, September, and October through June) soil N; 2.) plant species composition and ANPP; 3.) seasonal root and fungal dynamics in mini-rhizotrons; and 4.) soil temperature, moisture, and CO2 fluxes (using in situ solid state CO2 sensors). Also, soil N2O fluxes, predawn and mid-day (10-12 AM) water potential, and mid-day leaf photosynthetic gas exchange and stomatal conductance of black grama are measured prior to and up to 5 days after scheduled precipitation events.
Above-Ground Net Primary Productivity (ANPP) measurements:
Above-ground net primary production data is collected in the spring and fall. Spring measurements are taken in April or May when shrubs and spring annuals have reached peak biomass. Fall measurements are taken in either September or October when summer annuals have reached peak biomass but prior to killing frosts.
Vegetation data is collected on a palm top computer. A 1-m2 PVC-frame is placed over the fiberglass stakes that mark the diagonal corners of each quadrat. When measuring cover it is important to stay centered over the vegetation in the quadrat to prevent errors caused by angle of view (parallax). Each PVC-frame is divided into 100 squares with nylon string. The dimensions of each square are 10cm x 10cm and represent 1 percent of the total area.
The cover (area) and height of each individual live (green) vegetative unit that falls within the one square meter quadrat is measured. A vegetative unit consists of an individual size class (as defined by a unique cover and height) of a particular species within a quadrat. Cover is quantified by counting the number of 10cm x 10cm squares filled by each vegetative unit. It is possible to obtain a total percent cover greater than 100% for a given quadrat because vegetative units for different species often overlap.
Niners and plexidecs are additional tools that can help accurately determine the cover a vegetative unit. A niner is a small, hand-held PVC frame that can be used to measure canopies. Like the larger PVC frame it is divided into 10cm x 10cm squares, each square representing 1% of the total cover. However, there are only nine squares within the frame, hence the name “niner.” A plexidec can help determine the cover of vegetative units with covers less than 1%. Plexidecs are clear plastic squares that are held above vegetation. Each plexidec represents a cover of 0.5% and has smaller dimensions etched onto the surface that correspond to 0.01%, 0.05%, 0.1%, and 0.25% cover.
It is extremely important that cover and height measurements remain consistent over time to ensure that regressions based on this data remain valid. Field crew members should calibrate with each other to ensure that observer bias does not influence data collection
Grasses-To determine the cover of a grass clump, envision a perimeter around the central mass or densest portion of the plant, excluding individual long leaves, wispy ends, or more open upper regions of the plant. Live foliage is frequently mixed with dead foliage in grass clumps and this must be kept in mind during measurement as our goal is to measure only plant biomass for the current season. In general, recently dead foliage is yellow and dead foliage is gray. Within reason, try to include only yellow or green portions of the plant in cover measurement while excluding portions of the plant that are gray. This is particularly important for measurements made in the winter when there is little or no green foliage present. In winter, sometimes measurements will be based mainly on yellow foliage. Stoloniferous stems of grasses that are not rooted should be ignored. If a stem is rooted it should be recorded as a separate observation from the parent plant.
Forbs-The cover of forbs is measured as the perimeter of the densest portion of the plant. If the forb is an annual it is acceptable to include the inflorescence in this measurement. If the forb is a perennial, do not include the inflorescence as part of the cover measurement. Measure all foliage that was produced during the current season, including any recently dead (yellow) foliage. Avoid measuring gray foliage that died in a previous season.
Cacti-For cacti that consist of a series of pads or jointed stems (Opuntia phaecantha, Opuntia imbricata) measure the length and width of each pad to the nearest cm instead of cover and height. Cacti that occur as a dense ball/clump of stems (Opuntia leptocaulis) are measured using the same protocol as shrubs. Pincushion or hedgehog cacti (Escobaria vivipara, Schlerocactus intertextus, Echinocereus fendleri) that occur as single (or clustered) cylindrical stems are measured as a single cover.
Yuccas-Make separate observations for the leaves and caudex (thick basal stem). Break the observations into sections of leaves that are approximately the same height and record the cover as the perimeter around this group of leaf blades. The caudex is measured as a single cover. The thick leaves of yuccas make it difficult to make a cover measurement by centering yourself over the caudex of the plant. The cover of the caudex may be estimated by holding a niner next to it or using a tape measure to measure to approximate the area.
Height is recorded as a whole number in centimeters. All heights are vertical heights but they are not necessarily perpendicular to the ground if the ground is sloping.
Annual grasses and all forbs-Measure the height from the base of the plant to the top of the inflorescence (if present). Otherwise, measure to the top of the green foliage.
Perennial grasses-Measure the height from the base of the plant to the top of the live green foliage. Do not include the inflorescence in the height measurement. The presence of live green foliage may be difficult to see in the winter. Check carefully at the base of the plant for the presence of green foliage. If none is found it may be necessary to pull the leaf sheaths off of several plants outside the quadrat. From this you may be able to make some observations about where green foliage is likely to occur.
Perennial shrub and sub-shrubs-Measure the height from the base of the green foliage to the top of the green foliage, ignoring all bare stems. Do not measure to the ground unless the foliage reaches the ground. Plants rooted outside but hanging into a quadrat-Do not measure the height from the ground. Measure only the height of the portion of the plant that is within the quadrat.
Recording the Data:
Excel spreadsheets are used for data entry and file names should begin with the overall study (npp), followed by the date (mm.dd.yy) and the initials of the recorder (.abc). Finally, the site abbreviation should be added (i.e., c, g, b, p). The final format should be as follows: npp_mons.mm.dd.yy.abc.xls. File names should be in lowercase.
August 2009 Burn:
On August 4, 2009, a lightning-initiated fire began on the Sevilleta National Wildlife Refuge. The Monsoon site was entirely burned on this date, with all plots subjected to fire of comparable intensity.
01/13/2011-Spring and Fall 2010 data was updated, QA/QC'd and uploaded. (JMM) 11/26/2009-Spring 2009 data was updated, QAQC'd and uploaded. Fall season data was not collected due to an unexpected fire at Sevilleta LTER in Aug 2009. (YX) 11/26/2009-Metadata complied for data from 2006 to 2009. (YX) 01/14/2009-Metadata compiled for data from 2006 to 2008. (YX) 12/20/2008-Data was QAQC'd in MySQL. I checked for duplicates, missing quads, and species codes. (YX)
Other researchers involved with collecting samples/data: Chandra Tucker (CAT; 04/2014-present), Megan McClung (MAM; 04/2013-present), Stephanie Baker (SRB; 09/2010-present), John Mulhouse (JMM; 08/2010-06/2013), Amaris Swann (ALS; 08/2008-01/2013), Maya Kapoor (MLK; 08/2003 - 01/2005, 05/2010-03/2011), Terri Koontz (TLK; 02/2000 - 08/2003, 08/2006 - 08/2010), Yang Xia (YX; 01/2005 - 03/2010), Karen Wetherill (KRW; 02/2000 - 08/2009); Michell Thomey (MLT; 09/2005 - 08/2008).Data updated 08/18/15: MOSQ changed to MUSQ3; ARPUP6 changed to ARPU9; SPWR changed to SPPO6.
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