organic matter

Bosque Ecosystem Monitoring Program

The late Dr. Clifford S. Crawford established the Sevilleta’s Schoolyard LTER Program which funds an educational outreach program known locally as the Bosque Ecosystem Monitoring Program (BEMP).  The major focus of the program is to monitor key indicators of structural and functional change in the Middle Rio Grande riparian cottonwood forest ('bosque') corridor through central New Mexico, including the Sevilleta National Wildlife Refuge.

Ecological Effects of Prescribed Fire in a Chihuahuan Desert Grassland at the Sevilleta National Wildlife Refuge, New Mexico (2003- )

SEV_ID: 
146
Dataset ID: 
146
Abstract: 

Fire resulting from natural ignition has become a more common event on the Sevilleta National Wildlife Refuge (NWR) since the exclusion of domesticated livestock. Efforts to return fire to the native landscape has resulted in the use of prescribed fire during periods that meet burn prescriptions. A prescribed fire was performed on the Sevilleta NWR in June 2003. Among the measured site and burn characteristics that were measure, this project sampled soils before and after the fire from 5 previously-sampled locations that were burned in June 2003 and from 5 newly established locations that served as controls. The controls were within an area that was sampled between 1989 and 1996 for similar properties measured in this study and had previously been tested to be similar to the locations burned in 2003. The soil properties that are repeatedly measured at the burn and control locations include: field water content; water-holding capacity; organic matter; field extractable nitrate and ammonium; and potentially mineralizable nitrogen.

Keywords: 
fires
measurements
water content
temperature
incubation
burning
ammonium
nitrate
water
soil
grasses
Additional Information: 

Additional Study Area Information

Study Area Name: Bridge 1.1

Study Area Location: north end of five bridges; black grama dominated grassland; MacKensie Flats; Site is 5 m area around bridge; Bridges setup in 1994 to monitor changes in soil surface elevations to understand the dynamics of soil particles and associatednutrients.

North Coordinate: 34.3358
South Coordinate: 34.3358
East Coordinate: -106.6954
West Coordinate: -106.6954

Maintenance: 

Changes to the data: Data were updated to include 2007 data on 5/15/2008 by Carl White.

Personnel Involved
Data Manager: 
Geo Temporal Info
Publication Date: 
03/01/2011
Site: 
Date Range: 
Wed, 06/11/2003 - Wed, 10/17/2007
Methods
Methods: 

Bridges

The removable bridge is placed upon the end rebar and the middle pin is secured in the depression on the nail beneath the middle hole (#16). The bridge is then leveled and individual pins are inserted to the soil surface. If the surface is firm enough, the pins are left unsecured. If the surface is too soft, the pins are secured with the tip at the soil surface by attaching a clothspin above the bridge. The heights of each pin above the bridge are recorded, and cover is recorded if the pin struck vegetation when being inserted and basal cover is recorded if the pin rested upon the basal portion of a plant at the ground surface. The standard soil bridge developed for the Sevilleta was used. The bridge contains 31 holes at 5 cm intervals with the middle hole used to orientate the bridge above a nail left at the ground surface, and which provides a reference to secure the line and the bridge height. Also referenced at (http://sevilleta.unm.edu/data/contents/SEV065/).

Soil Collection

For inorganic N extractions and potentially mineralizable N measurements, a soil core of 4-cm diameter was taken to 20-cm depth beneath two nearby grass clumps (the two cores were compostited; termed under) and from two bare soil patches (two cores were composited; termed open) within 5 m of the stake identifying each bridge or from the bridge stake with the identification tag (new control bridges). All soil samples were placed into an ice chest and transported on ice directly to the University of New Mexico UNM, where they were sieved (< 2 mm), mixed, and stored at 5 degrees C. All soil N measurements were performed at UNM.

Soils Analysis

After determining fresh water content and water-holding capacity (WHC)(White and McDonnell 1988), fresh portions of each sample were adjusted to 50% of determined WHC and subsamples of 20 g dry-weight were apportioned into plastic cups. One subsample of each sample was immediately extracted with 100-ml 2 N KCl for NH4+-N and NO3-N analyses to determine field-available N. Two additional cups were covered with plastic wrap, sealed with a rubber band, and incubated in the dark at 20 degrees C. The plastic wrap minimized water loss during incubation, yet exchange of CO2 and O2 was sufficient to keep the subsamples aerobic during incubation. Moisture content was monitored by mass loss and replenished as needed. After contact and settling for 18-24 h, the clarified KCl was filtered through a Kimwipe and analyzed for NH4+-N and NO3--N+NO2--N on a Technicon AutoAnalyzer (Technicon, Terrytown, NY) as described in White (1986). After incubation for 6 weeks, a subsample of each soil was extracted with KCl and analyzed for NH4+-N and NO3--N+NO2--N. Potentially mineralizable N was determined to be the amount of extractable N in the 6-week extraction.

Organic Matter

Organic matter was determined by loss-upon-ignition in tin cups following heating at 500C for two hours.

Soil Physical/Chemical Properties

Soil cores were taken beneath grass clumps in which the temperature pellets were placed both before and after the fire. At least 300 g of soil were taken to a depth of 10 cm (NOTE:different depth than nitrogen cycling)

Fire Temperature

Fire temperature was determined with pellets supplied by Tempil (2901 Hamilton Blvd., South Plainfield, NJ 07080; www.tempil.com). A set of 15 foil-wrapped tablets, with melting temperatures ranging from 85 C to 1533 C, were strungon wire and suspended about 1 inch above the ground. The fire temperature was assumed to be greater than the temperature at which the corresponding pellet showed signs of melting and less than the temperature of the next highest undamaged pellet. The pellets were suspended within two clumps of dominant grasses at the site (black grama).

Pre-existing briges (1.1 through 1.5) were selected to be included within a prescribed burn area. Data collected from the bridges were consistent with existing data collection: (http//sevilleta.unm.edu/data/contents/SEV065/ ) and included soil surface elevation, plant aboveground cover and basal cover. Soils from beneath a nearby grass clump and from bare interspaces were collected for analysis of soil properties. Soil temperature pellets were placed within grass clumps from beneath which soils were collected. Pre-fire on control and expected burn plots, and post-fire on burn plots only for soil elevation, aboveground plant cover and basal cover, N mineralization potentials, field moisture, water holding capacity, and loss upon ignition for organic matter. Pre and post burn soil samples were collected beneath grass clumps at the existing bridges for analysis of soil properties (sent ot Jane Belnap). Fire temperature was measured with temperature tablets placed about 1 cm above the ground within the grass clump that was sampled for soil properties and in an adjacent grass clump of similar appearance.

Litter Fall Collection Study in Pinyon-Juniper, Cottowood, and Spruce-Fir-Aspen Forests at the Sevilleta NWR, Bosque del Apache NWR, and the Cibola National Forest, New Mexico (1992-1993)

SEV_ID: 
22
Dataset ID: 
22
Abstract: 

The litterfall study was designed to assess the quantity of biomass (leaves, twigs, reproductive materials) falling from tree species in different ecosystem types. Three study sites selected were:  (1) the pinyon-juniper woodland site near Cerro Montoso on the Sevilleta NWR; (2) the cottonwood forest LTER site along the Rio Grande at Bosque del Apache NWR; and (2) the old-growth spruce-fir-aspen site near South Baldy in the Magdalena Mountains (Cibola National Forest).  The study was conducted over two years (1992-1993) to compare litterfall rates and quantities among sites, seasons and years.

Keywords: 
habitats
species
biology
biomass
aboveground biomass
biogeochemical processes
decomposition
leaf decomposition
litter decomposition
biological processes
litterfall
production
terrestrial ecosystems
forest ecosystems
old growth forests
plants
trees
vegetation
leaf litter
Purpose: 

To assess differences in rates and quantities of leaf, twig, reproductive parts (nuts, seeds, berries) of litterfall from tree species in various ecosystems studied by the Sevilleta LTER Program.

Additional Information: 

 

Magdalen Mountains Site, Cibola National Forest:

- Soil: rocky soils, with high organic matter (litter) below tree canopies.

- Slope/Aspect:  Various, ranging from flat to 30 degrees.  Easterly aspect.

- Vegetation Community: Mixed-species conifer forest with aspens.

- Terrain/Physiography: Mountainous

- Geology/Lithology:  Magdalena Mountains, derived from volcanic activity.

- Hydrology - surface/groundwater:  Virtually no runoff during storms due to deep litter layer.

- Size:  sampling area covered approximately 1 ha

- History (if known):  Old growth forest, no logging and no fire history.

- Elevation: 3,243 meters at weather station (station #46).

- Climate (general):  A summary of the meteorological data from the Langmuir Laboratory weather station in the Magdalena Mountains is shown below. For further climate details and data, consult the Sevilleta Meteorology databases.

Personnel Involved
Data Manager: 
Lab Crew: 
Geo Temporal Info
Date Range: 
Thu, 07/09/1992 - Wed, 09/15/1993
Methods
Methods: 

 

Sampling Design:

 A total of 120 litterfall baskets were distributed among the 3 study sites.  In the Sevilleta's Cerro Montoso site, 30 baskets were placed under juniper trees, and 30 were placed under pinyon trees.  At the Bosque del Apache site, 30 baskets were placed in 3 transect lines of 10 baskets each, at 10 meter intervals, through the cottonwood forest.  At the Magdalena Mountain site, 30 baskets were placed in 3 transect lines of 10 baskets each, at 10 meter intervals (as in the Bosque site).

Sample Unit:

Each basket was considered a sample unit. 

Frequency of Sampling:

During the same times as arthropod pitfall collections (several times/year)

Sample Size:

120 baskets total.  Each basket was a rubber basin with a small (5 mm) hole drilled in the bottom to allow rainwater and snowmelt to drain out. Basket dimensions were circular, with a diameter of 41.5 cm at the top rim, tapering inward to a diameter of 35 cm at the basket bottom.  The basket height was 12.5 cm.

Measurement Techniques:

Litterfall baskets were place under tree canopies to catch falling leaves, twigs, and reproductive parts.  In the Bosque del Apache and Magdalena Mountain sites, the baskets were placed systematically in 3 transect lines through the forest (which generally had a closed canopy).  In the Pinyon-Juniper site on SNWR, the baskets were placed under individual trees (basket locations were halfway between the trunk and the edge of the canopy) due to the patchiness of tree locations. Hence, the results for this site are for a "per tree" basis, and should be scaled up to reflect different tree densities in various Pinyon-Juniper sites.

Litterfall samples were collected by placing all litter into plastic zip-lock bags, marking each bag with the basket tag number, and taking the bags back to the laboratory. If samples were wet from rain/snowfall, the sample bags were opened and allowed to air-dry for several days.  Litter was then sorted by category (leaf, twig, reproductive part, or "miscellaneous" if the part could not be recognized) and by species.  The sorted litter was then oven dried at 60 degrees Centigrade for one week, and weighed on a Mettler top-loading balance.

Long-term Intersite Decomposition Team (LIDET) Plant Litter Data at the Sevilleta National Wildlife Refuge, New Mexico (1990-2001)

SEV_ID: 
35
Dataset ID: 
35
Abstract: 

Sevilleta data from a study testing the degree to which substrate quality and macroclimate control the carbon and nitrogen dynamics of decomposing leaf, wood, and fine-root litter in a 10-year, 28 site (17 LTER) team experiment.

http://andrewsforest.oregonstate.edu/data/abstract.cfm?dbcode=TD023&topnav=97

Keywords: 
biology
measurements
chemical properties
carbon to nitrogen ratio
field methods
decomposition
leaf decomposition
litter decomposition
ecosystems
terrestrial ecosystems
organisms
plants
grasses
herbs
Personnel Involved
Owner: 
Geo Temporal Info
Date Range: 
Mon, 01/01/1990 - Fri, 01/01/1999

Microbial Processes

Ultraviolet treatment screen for photodegradation study featuring litterbags and removable plastic louvers that block or pass UV radiation.
Investigators: 
Sinsabaugh, Robert

Most recent studies of microbial processes and microbial ecology at the Sevilleta National Wildlife Refuge (SNWR) have focused on the semiarid grassland biome. These studies can be broadly classified into process, phylogenetic and modeling categories.

Ultraviolet treatment screen for photodegradation study featuring litterbags and removable plastic louvers that block or pass UV radiation.

Monsoon Rainfall Manipulation Experiment (MRME)

Monsoon Rainfall Manipulation Experiment (MRME), Facing east. Taken July 10, 2007 by Mike T. Friggens.
Investigators: 
Collins, Scott

The Monsoon Rainfall Manipulation Experiment (MRME) enables the quantification of changes in the structure and function of a semiarid grassland ecosystem that have been caused by increased rainfall variability.  Variability in precipitation affects the timing and duration of the pulses of soil moisture that drive primary productivity, community composition, and ecosystem processes in semiarid grasslands.

Schematic of the on-the-ground layout of the Monsoon Rainfall Manipulation Experiment (MRME).

Plant Litter Decomposition at the Sevilleta National Wildlife Refuge, New Mexico (1990-1998)

SEV_ID: 
12
Dataset ID: 
12
Abstract: 

The long-term goal of the decomposition study was to document the effects of climate variation on decomposition of major plant litter-types. The project began in 1989 and underwent changes of locations and litter types. The long-term litter types included black grama, Indian rice grass, juniper, and creosote.  Mass loss of the litter types can be compared to precipitation and other meteorological factors obtained at nearby locations.

Keywords: 
species
biology
ecology
measurements
biomass
aboveground biomass
terrestrial
methods
field methods
litterbags
biological processes
litterfall
ecosystems
terrestrial ecosystems
organisms
plants
grasses
shrubs
trees
vegetation
leaf litter
Additional Information: 

 

Site Name:: Location 1, Black Butte

Site Location: SW of gate on east side of black butte (north border of east side)

Site Coordinates: 34.40667735, -106.68647480, NAD83

Site Size: 5 x 5 m

Site Elevation: 1560.2 m

Site Soil: sandy

 

Site Name:: Location 2, Deep Well

Site Location: deep well, east side

Site Coordinates: 34.35277814, -106.69230409, NAD83

Site Size: 5 x 5 m

Site Elevation: 1605.07 m

Site Vegetation: black and blue grama

 

Site Name:: Location 3, Old 5 points

Site Location: 1 mile east-southeast of 5 points

Site Coordinates: 34.27395094, -106.67859413, NAD83

Site Size: 5 x 5 m

Site Elevation: 16.92.34 m

 

Site Name:: Location 4, Larrea

Site Location: between location 3 and the south boundary

Site Coordinates: 34.24100599, -106.74927778, NAD83

Site Size: 5 x 5 m

Site Elevation: 1617.05 m

Site Vegetation: Creosote

 

Site Name:: Location 5, Ocotillo

Site Location: Near south boundary

Site Coordinates: 34.22190529, -106.70410020, NAD83

Site Size: 5 x 5 m

Site Landform: south facing slope

Site Elevation: 1723.05 m

Site Vegetation: Ocotillo

 

Site Name:: Location 6, Sepultura Canyon

Site Location: Sepultura Canyon

Site Coordinates: 34.30220417, -106.62011595, NAD83

Site Size: 5 x 5 m

Site Landform: foothills of the Los Pinos

Site Elevation: 1872.44 m

Site Vegetation: grass-juniper savannah

 

Site Name:: Location 7, Cerro Montoso

Site Location: Cerro Montoso

Site Coordinates: 34.36851996, -106.53503075, NAD83

Site Size: 5 x 5 m

Site Elevation: 1970.74 m

 

Site Name:: Location 8, Bosque del Apache

Site Location: Bosque del Apache NWR, east side of Rio Grande

Site Coordinates: 

Site Size: 5 x 5 m

Site Vegetation: riparian forest

 

Site Name:: Location 9F, Magdelena Mountains Forest

Site Location: Magdelena Mountains, west of Socorro

Site Coordinates: 33.98152914, -107.18597909, NAD83

Site Size: 5 x 5 m

Site Elevation: 3187.6 m

Site Vegetation: High elevation forest-meadow

 

Site Name:: Location 9M, Magdelena Mountains Meadow

Site Location: Magdelena Mountains, west of Socorro

Site Coordinates: 33.99204766, -107.17438462, NAD83

Site Size: 5 x 5 m

Site Elevation: 3033.6 m

Site Vegetation: High elevation forest-meadow

 

Site Name:: Location 10, Rio Salado

Site Location: Rio Salado

Site Coordinates: 34.29572804, -106.92662418, NAD83

Site Size: 5 x 5 m

Site Elevation: 1509.54 m

Site Soil: sandy soil

Site Vegetation: Chihuahua desert with creosote dominant

 

Site Name:: Location 11, Red Tank

Site Location: Red Tank, in foothills of ladrone Peak

Site Coordinates: 34.39791210, -107.03647141, NAD83

Site Size: 5 x 5 m

Site Elevation: 1767.12 m

Site Vegetation: Great Basin grass-shrub

 

Site Name:: Location 12, Blue Springs

Site Location: Blue Springs, (lower goat draw), northeast corner of SNWR

Site Coordinates: 

Site Size: 5 x 5 m

Site Vegetation: grass-juniper savannah

 

Site Name:: Location 13, 5 points

Site Location: east of actual road junction near site of grassland-creosote webs

Site Coordinates: 34.33272200, -106.73100528, NAD83

Site Size: 5 x 5 m

Site Elevation: 1613.89 m

Site Vegetation: creosote

 

Description of Initial Study: 

The decomposition study began with litter grown during 1989, which was harvested in the fall of1989, prepared during the winter and placed in the field the following spring. The initial study was designed by Dr. J. Gosz and Dr. R. Parmenter with C.S. White the project manager. The basic design included placement of three (3) primary litter-types (black grama (Bouteloua gracilis), juniper (Juniperus monosperma), and Indian       rice grass (Oryzopsis hymenoides)) at seven (7) locations. The seven locations included: along an approximate north-south transect from grass habitat to creosote habitat, Location 1 = Black Butte; Location 2 = Deep Well; Location 3 = 1 mi. east of 5 points (central point along the transect representing a grass-juniper-creosote junction); Location 4 = between Location 3 and south boundary within a creosote stand (Larea); and Location 5 = south boundary at a stand of Ocotillo (Ocotillo); and along an approximate east transect from location 3; Location 6 = Sepultura Canyon; and Location 7 = Cerro Montoso (increasing favorable juniper habitat and into pinyon). There were two other locations off the Sevilleta NWR in the first year: Location 8 at the Bosque del Apache (which later was lost during a fire at that location); Location 9 in the Magdelena Mountains west of Socorro.

At all locations (except Bosque del Apache), litter of the three common species were included. Litter of different species were placed at locations where that litter may be dominant. The other litter types included: creosote (locations 2 and 4), blue grama (location 2), 4-wing saltbush (location 2), yucca (location 2), pinyon (location 7), cottonwood (location 8); and Arizona fescue, Douglas fir, Ponderosa pine, and aspen (location 9).

At each location and for each species, the experimental design included placement of enough bags for three replicate bags per collection date; one collection at the time of placement and eight additional collections over a two year period, and 7 additional bags to allow for some mortality of bags (34 bags total). Each bag initially contained 5.00 g +/- 0.05 g (4.95-5.05g) air-dried material. Bags were placed at each location in late February, 1990. Collections are projected to be made in: (1), May 1990 (2), July 1990 (3), Sept. 1990 (4), January 1991 (5, one-year), May 1991 (6), Sept 1991 (7), and January 1992 (8, two-year).

Changes in 1990: 

In 1990, litter was only placed at the Deep Well location (#2) and only litters of black grama, juniper, rice grass, creosote, blue grama and saltbush were used. It was decided that all the sites were not worth continuing because there were no other data associated with the site that could be used to explain why decomposition may or may not vary at that site versus and other site. Thus, the location with the most complete meteorological data was maintained (Deep Well, location 2).

Changes 1991 through 1998: 

Starting with litter collected in the fall of 1991 and continuing through litter collected in 1997, litter bags were placed at four (4) locations that represented the range of climates present on the Sevilleta and that were all near meteorological stations. Deep Well (location 2) and Cerro Montoso (location 7) were retained from the previous work because they were near meteorological stations. Cerro Montoso (location 7) represented a      pinyon-juniper forest, upper elevation climate (wettest of all locations), Deep Well (location 2) represented a short-grass prairie climate, a location near the Rio Salado (new, location 10) represented a Chihuahuan desert climate (driest of all locations), and Red Tank (new, location 11) represented a Great Basin grass-shrub climate. Deep Well and Cerro Montoso (location 1 and 7, respectively) are on the east side of the Sevilleta while Rio Salado and Red Tank (locations 10 and 11, respectively) are on the west.

At these four locations, black grama, creosote, Indian rice grass, and juniper litter were placed every spring. Blue grama litter also was placed at Deep Well to maintain a long-term blue and black grama comparison.

Changes in 1998: 

Beginning with placement of litter collected in the fall of 1998, efforts to conserve resources and to address changes across vegetation transition zones lead to addition of 2 new locations: Blue Springs (location 12), a juniper-short grass prairie mixture; and 5 points (location 13), a creosote area near the Deep Well short grass-desert grass area. No new litter was placed at Rio Salado and Red Tank (locations 10 and 11), but remaining     litter were collected for the 1 year decomposition measurement in Feb. of 1999 and will be collected again in Feb. of 2000 for the two year decomposition measurement. The four common litter types were placed at Cerro Montoso, Blue Springs, Deep Well, and 5 points (locations 7, 12, 2, and 13, respectively) with blue grama also at Deep Well.

 

Study Personnel: James R. Gosz; Carl White; John Craig; Doug Moore; John Dewitt; Todd Haagenstad; Lisa Apodoca; Erica Barner; Micky Boise; Kavita Patel; Steve Hofstad, Tze Sun Yong; Luis Guzman; Chris Thomas; David Wales; Kerry Carr; Deb Sena; Olivia Hopkins 

Personnel Involved
Owner: 
Associated Researcher: 
Geo Temporal Info
Date Range: 
Fri, 02/23/1990 - Thu, 02/25/1999
Methods
Methods: 

Experimental Design:  

Setting up each location: 1989 through 1991:

The design of this decomposition study includes placement of three (3) primary litter-types (black grama, juniper, and indian rice grass) at seven (7) locations. Litter of each species was collected after senescence in the fall. The grasses were clipped from standing plants before the litter was on the ground. All material that was not produced during the previous growing season was discarded.  All reproductive parts were discarded. If there was any doubt about whether or not the material was produced that growing season, it was discarded. Juniper was collected from trees with senescent material or that had recently died and were still on the branch. Juniper litter consisted of only that material that still had bracts (woody stems without bracts were discarded). Pinyon was collected from trees that still retained senescent needles by shaking and capturing on a cloth or plastic sheet.  Branches of 4-wing saltbush were trimmed from bushes in October when material looked senescent and leaves were picked from the branches and air-dried. Creosote leaves were attempted to be collected by spreading sheets on the ground; however, litterfall is very episodic and not enough material could be collected. Thus, creosote was collected by cutting live plants and drying in a forced-air oven at 60 °C; then the freshly dried leaves were removed from the branch and any reproductive parts (seeds) were discarded. Yucca was collected by cutting leaves from a recently dead yucca plant. Cottonwood leaves were collected in plastic trays as they fell and before the fresh litter was rained on. Arizona fescue, douglas fir, ponderosa pine and aspen were collected by others and we do not know what methods were used.  All litters were sorted and damaged material or reproductive parts were discarded. Unless previously dried, litter was air-dried in the laboratory. Litter bags consisted of coated fiberglass window screen material cut into 12 by 7.5 sheets, which were folded in half and two of the edges folded over and stapled. Litter was inserted through the unstapled edge, which then was folded over and stapled. Each bag initially contained 5.00 g (4.95-5.05 g) of air-dried material. For each litter type, a total of 34 bags were prepared for placement at each location. All the litter bags of one species for an individual location were placed in a separate bag and the bag was sealed for transport the field.

At each location and for each species, the experimental design included placement of enough litter bags for three replicate bags per collection date; one collection at the time of placement, eight collections over a two year period, and 7 additional bags to allow for some mortality of bags (34 bags total per location).

When the litter bags were placed at each location, the location was marked with fence posts as a warning. Each location was identified by a color-coded marker. The color-coded markers for each location are as follows: location (1)=plain, (2)=tan, (3)=green, (4)=orange, (5)=orange/green, (6)=tan/green, (7)=green/tan, (8)=green/orange, and (9)=tan/orange.

Litter bags were placed on bare soil between plants at all times. Each litter bag was anchored by inserting a 16 penny nail through diagonal corners of the bag and into the ground.  Decomposition bags with creosote, blue grama, yucca (tethered, not bagged), and four-wing saltbush all were placed at the deep well location (location 2); and cottonwood at the Bosque del Apache (location 8).

In the fall of 1990, only 5 litter types (black grama, blue grama, juniper, creosote, and Indian ricegrass) were collected for placement in Feb. of 1991. Blue grama litter was only placed       at Deep Well, while the four other litter types were place at 4 locations (#2, 7, 10, 11). Litter was collected as described in the previous year and bags were placed on bare soil at each location.

In February of 1999, the Rio Salado and Red Tank sites did not get new litter bag placements; instead the new locations at Blue Springs and 5 points were established and the 4 common litter types were placed at these locations along with Deep Well and Cerro Montoso.

Sample Collection Methods:

For each individual location collection, three replicate bags are placed in zip-lock bags and are identified by species, field location, collector initials, and date of collection. A general maintenance survey of each location is done at this time by the collector(s). At the time of placement, three bags of each species were collected and placed into a gallon-sized zip-loc bag for transport to the lab. This method insured that each replicate was handled the same way with bouncing during transport and sample handling consistent for all samples. These three samples taken at the time of placement determine the starting (incubation in the field time 0) replicate litters. For each collection date, 28 gallon-size zip-lock bags were be needed.

Sample Analysis Methods:

Overview: 

Handling of the field samples involves three phases: (1) initial cleaning and oven-dry weight; (2) grinding and ash correction; and (3) chemical analyses. Once in the laboratory, field samples will come in with 3 replications/plant type in a bag labelled with the site, plant material, and collection date. In Phase 1, the samples are cleaned, oven dried at 60° C for a minimum of 24 hours, and oven-dry weight recorded. The  samples are then transferred to coin envelopes, ground on the Tecator grinder, and stored back in the coin envelope. In Phase 2, ground plant material is then used for ash-free weight determinations. In Phase 3, the remaining ground plant litter is used for chemical analyses.

Phase 1. 

WEIGHING 

1.  A zip-loc bag with field samples  is selected to begin weighing out. One mesh bag is selected and  this sample was arbitrarily assigned as a replicate number and Sample ID number. Any foreign material was removed from the outside of the bag, such as: differen plant material, mud, ROCKS, etc, making sure to lift up the side folds to release any trapped rocks.  The weighed and labelled weigh boat was placed on a sheet of paper and the contents of the bag were emptied into the weigh boat. This was repeated for all samples and the samples were placed in the oven. 

2.  Samples were dried for a minimum of 24 hours at 60° C. Samples were weighed and weights were recorded in the record book.  Samples were then placed into a coin envelope labelled with the collection date, site number, plant type, and Sample ID number.

GRINDING 

Plant material was ground in order to perform ash-free weight corrections and chemical  analyses. The only plant material needed to be ground using liquid N were: pinyon, juniper and creosote. All others were ground warm.

Phase 2. 

ASH-FREE WEIGHT CORRECTION METHOD 

Methods 1989 through 1991: 

ASH THE EMPTY CRUCIBLES: Line up a sufficient quantity (about 40) of the tall, narrow, numbered porcelain crucibles. Wipe them out with a Kimwipe. Load them into the muffle furnace making sure not to touch the oven sides or each other. Use the shelf to fit them all in. Close the muffle furnace. Turn on the exhaust fan. Turn the controller to 5.0, and turn the timer to 2 hours. After about 1.5 - 1.75 hours, check to verify that the temperature has or will reached 500 °C.  If it hasn't, add additional time to the controller. When it does hit 500 °C, it is hot enough and ready to shut off. Let cool, closed, overnight. The next day the crucibles can be removed and stored in a desiccator.

DRY THE GROUND MATERIAL: The ground material in their envelopes  was placed in a 60 °C oven for 24 hours prior to weighing out for ashing. When the crucibles are thoroughly cooled, weigh the empty crucible and then place out approximately 1 gram using the analytical balance (BE SURE TO MIX THE SAMPLE WELL BECAUSE PARTICLES TEND TO SEPARATE DURING STORAGE AND HANDLING) of ground material into the crucible, and then record the filled crucible weight on the data charts in the front of this book. Remember to weigh and include a blank (empty) crucible with each run.

ASH THE WEIGHED SAMPLE:  Using tongs, load the crucibles into the muffle furnace, making sure not to touch the oven sides or each other. Use the shelf to fit them all in. Close the muffle furnace. Turn on the exhaust fan. Turn the controller to 5.0, and turn the timer to 2 hours. After about 1.5 - 1.75 hours, check to verify that the temperature has reached 500 °C. If it hasn't, add an extra 15 minutes to the timing. When it does hit 500°C, and the timer has turned off the furnace, turn the controller to 2.0, and the timer to 2 hours. Let cool, closed, overnight.

WEIGH THE ASHED SAMPLES: Using tongs, remove the crucibles to a desiccator. When they are thoroughly cooled, use the same analytical balance and record the filled crucible weight on the data charts in the front of this book. Remember to weigh and include the blank (empty) crucible from each run. Dump out the ashed sample into the garbage, wiping the crucible with a Kimwipe if necessary. The crucibles are now ready to be filled again and fired. 

Methods after 1991: 

Ashing methods were changed in 1991 when the use of porcelain crucibles was replaced by use of disposable aluminum boats. All methods stayed the same EXCEPT: The disposable aluminum boats did not need to be 'tared' or fired before use. The clean boats were taken directly from the package and placed into use. To identify the boat, the SAMPLE ID # was 'written' (etched indented with a pencil-pen) on the bottom of the boat. The boat weight was recorded and the sample (WITH MIXING!) was added (about 1 gram). The rest of the procedures remained the same. If the muffle furnace was allowed to exceed 550 °C, the aluminum boats would melt and significant changes in their weight could occur. Blank boats are run with each operation to insure no significant loss-gain during firing.

Analytical Methods Used:

Kjeldahl Nitrogen and Phosphorus by Technicon Industrial Method No. 369-75A (Revised 8/21/75) Digestion and sample preparation for the analysis of total kjeldahl nitrogen and/or phosphorus in food and agricultural products using the technicon BD-20 Block digestor and Technicon Industrial Method 334-74A (revised 8/21/75) Individual/simultaneous determination on nitrogen and phosphorus in BD acid digests.

Percent Nitrogen and Percent Carbon were determined by High Temperature combustion, the resulting gases were eluted on a gas chromatography column and detected by thermal conductivity and integrated to yield carbon and nitrogen content.  Analyses were performed on a ThermoQuest CE Instruments NC2100 Elemental Analyzer, ThermoQuest Italia S.p.A., Rodano, Italy.  

Instrumentation: 

Study Instrumentation: ThermoQuest CE Instruments, NC2100, Elemental Analyzer (Nitrogen and Carbon).

Sevilleta LTER Vegetation Sample Catalog- Ground Samples for Chemical Analysis (2000-2013 )

SEV_ID: 
294
Dataset ID: 
294
Abstract: 

Several long-term studies at the Sevilleta LTER measure net primary production (NPP) across ecosystems and treatments. Net primary production is a fundamental ecological variable that quantifies rates of carbon consumption and fixation. Estimates of NPP are important in understanding energy flow at a community level as well as spatial and temporal responses to a range of ecological processes. The NPP weight data (SEV 157) is obtained by harvesting a series of covers for species observed during plot sampling. These species are always harvested from habitat comparable to the plots in which they were recorded. This data is then used to make volumetric measurements of species and build regressions correlating biomass and volume. From these calculations, seasonal biomass and seasonal and annual NPP are determined.  These sampled are then vouchered for use to do analyses of inorganic and organic components such as carbon, nitrogen, and phosphorous as well as and other macro and micro nutrients and organic components such as cellulose and lignin.    

Keywords: 
species
plant species
measurements
productivity
net primary productivity
annual net primary production
primary production
net primary production
harvesting
organisms
plants
Personnel Involved
Data Manager: 
Lab Crew: 
Geo Temporal Info
Date Range: 
Mon, 10/11/1999 - Wed, 10/24/2012
Methods
Methods: 

After all aboveground net primary production (ANPP) quadrat measurements are complete, plants of similar size classes are harvested outside the permanent quadrats.  These samples are sorted, dried, and weighed and the resulting data (weight dataset- SEV157)  is used to create regressions that estimate aboveground biomass.  Then the harvest samples of all size classes, for each species, are then combined to make a voucher sample. A subsample of that combined sample is then ground up mechanically and stored in a sealed glass vial. These samples are available for quantitative chemical analysis of their inorganic and organic composition.  Seasonal as well as inter-annual compositions of the various species on the Sevilleta can be derived from this material.  The samples are stored at the Sevilleta Field Station.  Please contact Stephanie Baker for sample access.  

Small Mammal Exclosure Study (SMES) Leaf Litter Study in the Chihuahuan Desert Grassland and Shrubland at the Sevilleta National Wildlife Refuge, New Mexico

SEV_ID: 
96
Dataset ID: 
96
Abstract: 

This is data for cover of dead plant leaf litter accumulations on soil surfaces measured on each of the SMES study plots. Leaf litter cover was measured from each of the 36 one-meter2 quadrats twice each year when vegetation was measured.

Keywords: 
measurements
litter
roads
soil
deserts
grasslands
grasses
leaves
vegetation
leaf litter
Additional Information: 

Additional Information on the personnel associated with the Data Collection / Data Processing

Sevilleta Field Crew Employee History

Megan McClung, April 2013-present, Stephanie Baker, October 2010-Present, John Mulhouse, August 2009-Present, Amaris Swann, August 25, 2008-January 2013, Maya Kapoor, August 9, 2003-January 21, 2005 and April 2010-March 2011, Terri Koontz, February 2000-August 2003 and August 2006-August 2010, Yang Xia, January 31, 2005-April 2009, Karen Wetherill, February 7, 2000-August 2009, Michell Thomey, September 3, 2005-August 2008, Jay McLeod, January 2006-August 2006, Charity Hall, January 31, 2005-January 3, 2006, Tessa Edelen, August 15, 2004-August 15, 2005, Seth Munson, September 9, 2002-June 2004, Caleb Hickman, September 9, 2002-November 15, 2004, Heather Simpson, August 2000-August 2002, Chris Roberts, September 2001-August 2002, Mike Friggens, 1999-September 2001, Shana Penington, February 2000-August 2000.

Maintenance: 

SEVILLETA SMES LEAF LITTER DATA 1998 log Updat03/09/06 Status DONE 01/31/06 - Checked data for missing data points, doubles, and errors. Missing data points were recorded using -999 (Human Error) or -888 (-888), duplicates of data points were removed, and errors were corrected. If a data point contained a measurement and a measurement with a count, the zero observation was removed. - Removed Species, and Per fields. Tape field was changed to ID# and observations made in the Per field were moved to the new ID# field. No observations were made in the tape field. EC field was added and NA was recorded in this field for this year. Date MM/DD/YY field was changed to just DATE. Other changes in the fields include PLT to PLOT, BLK to BLOCK, and SPECICOV to COVER. - Any empty cells were filled in with -999 (or -888) for missing data or an NA for not applicable. - Yang Xia 02/24/06 - Checked data for missing data points, doubles, and errors. Missing data points were recorded using -999 (Human Error) or -888 (-888), duplicates of data points were removed, and errors were corrected. If a data point contained a measurement and a measurement with a count, the zero observation was removed. - Removed Species, Comments, and Per fields. Tape field was changed to ID# and observations made in the Per field were moved to the new ID# field. No observations were made in the tape field. EC field was added and NA was recorded in this field for this year. Date MM/DD/YY field was changed to just DATE. Other changes in the fields include PLT to PLOT, BLK to BLOCK, and SPECIcOV to COver. - In the spring, Missing all Plots for Block 1 at the Grassland site. There are missing data in Site G Block 2 Plot 3 Treatment C, Site G Block 3 Plot 3 Treatment R, and Site G Block 4 Plot 2 Treatment C. - For the fall, All plots are presented, but with several data points missing. - Any empty cells were filled in with -999 (or -888) for missing data or an NA for not applicable. - Yang Xia 03/03/06 - Changed data to fit parameters. For example if a measurement was 6 it was rounded to the closest appropriate value, which in this case is 5. See 'Variable Descriptions' Variable 7. Also, if a measurement was less than 0.5 it was rounded to 1 to indicate that an occurence was present. - Yang Xia 03/06/06 - Quads 21-24 were originally classified as Trt C in the spring at the Grass site for Blk 4 Plt 3, Changed to Trt L. - Quads 10-17 were originally classified as TRT C in the spring at the Creosote Site for BLOCK 2 PLOT 2, changed the TRT to TRT L. - Quads 11-14 were originally classified as TRT C in the spring at the Creosote Site for BLOCK 3 PLOT 4, changed TRT to TRT L. 03/08/06 - Modified metadata to correct format. - Yang Xia 03/09/06 - changed EC variable data to "1" with the comments, and "0" for no comments. NA for not applicable. 03/09/06 - changed start date from september 1995 to May 1995 in the research Hypotheses, since the data collection was starting on 05/02/95. doc

Personnel Involved
Owner: 
Data Manager: 
Geo Temporal Info
Publication Date: 
10/04/2010
Date Range: 
Mon, 05/08/1995 - Thu, 10/20/2005
Methods
Methods: 

Experimental Design:

There are 2 study sites, the Five Points grassland site, and the Rio Salado creosotebush site. Each study site is 1 km by 0.5 km in area. Three rodent trapping webs and four replicate experimental blocks of plots are randomly located at each study site to measure vegetation responses to the exclusion of small mammals. Each block of plots is 96 meters on each side. Each block of plots consists of 4 experimental study plots, each occupying 1/4 of each block. The blocks of study plots are all oriented on a site in a X/Y coordinate system, with the top to the north. Treatments within each block include one unfenced control plot (Treatment: C), one plot fenced with hardware cloth and poultry wire to exclude rodents and rabbits (Treatment: R), and one plot fenced only with poultry wire to exclude rabbits (Treatment: L). The three treatments were randomly assigned to each of the four possible plots in each block independently, and their arrangements differ from block to block. Each of the three plots in a replicate block are separated by 20 meters.

Each experimental measurement plot measures 36 meters by 36 meters. A grid of 36 sampling points are positioned at 5.8-meter intervals on a systematically located 6 by 6 point grid within each plot. A permanent one-meter by one-meter vegetation measurement quadrat is located at each of the 36 points. The 36 quadrats are numbered 1-36, starting with number 1 in the top left corner (north-west) of each plot (top being north), and running left (west) to right (east), then down (south) one row, and then right (east) to left (west), and so on Quadrat/rebar number one is in the northwest corner of each plot, and numbers 1-6 are across the north side of the plot west to east, then quadrat/rebar number 7 is just south of quadrat/rebar number 6, and rebar numbers increase 7-12 east to west, and so on. 3-inch nails were originally placed in the top left(north-west) corner of each quadrat. These may be difficult to see. A 3-meter wide buffer area is situated between the grid of 36 points and the perimeter of each plot.

While measuring vegetation on each quad, the total amount of leaf litter cover on the soil surface that was seen on each quadrat was measured. "Leaf litter cover(n)" for the cover of leaf litter on the soil surface of the quadrat was stated in terms of the 10 cm squares. For cover values less than 5, increments of 1.0 were used. For cover values greater than 5, increments of 5.0 were used. Leaf litter included all detached dead plant material on the soil surface, including woody branches. Only leaf litter cover that is in the open was measured, not within clumps of grass, etc. Some leaf litter cover had distinctive margins and was easy to define and measure. However, much leaf litter consisted of many diffuse small patches that are separated by bare soil, and distributed throughout the quadrat. For such diffuse cover, the actual cover in one typical 10 by 10 cm square (e.g., 0.3) was determined, the number of squares with diffuse cover (e.g., 5) was counted, then the number of squares was multiplied by the actual cover for a typical square (e.g., 0.3 X 5 = 1.5, then round to 1.0 or 2.0, or if the value had been greater than 5, round to the nearest increment of 5.0) for the total litter cover. All litter cover was pooled into one observation, and no height was measured.

Soil Characteristics Following a Lightning-Initiated Fire at MacKenzie Flats, Sevilleta National Wildlife Refuge, New Mexico, 1998

SEV_ID: 
170
Dataset ID: 
170
Abstract: 

We evaluated soil characteristics after a lightning-initiated fire. Following the fire in July 1998, 25 experimental plots were established on the eastern edge of MacKenzie Flats at the Sevilleta National Wildlife Refuge. Ten of these plots were located in a Bouteloua gracilis (blue grama)-dominated site, while 15 were established in another area dominated by Bouteloua eriopoda (black grama). All plots were oriented along a topographic gradient that ran in an east-west direction. At three topographic locations within each plot, soil samples were taken at two depths from an area covered by perennial grass as well as an area devoid of vegetation. Soil samples were collected in July 1998 and analyzed for moisture content and soil texture.

Keywords: 
disturbances
fires
wildfires
lightning
soil properties
soil texture
soil moisture
cores
sand
soil
deserts
grasslands
grasses
vegetation
Station Acronym: 
SEV
Personnel Involved
Contact: 
Data Manager: 
Lab Crew: 
Geo Temporal Info
Publication Date: 
10/04/2010
Site: 
Date Range: 
Wed, 07/01/1998
Methods
Methods: 

Experimental Design - Following a lightning-initiated fire in July 1998, 25 experimental plots were established on the eastern edge of the MacKenzie Flats area of the Sevilleta National Wildlife Refuge. Ten of these plots were located in a Bouteloua gracilis (blue grama)-dominated site, while 15 were established in another study area, where B. eriopoda (black grama) was more abundant. In the former site, five of the 10 plots were established in burned areas, and the others were positioned in unburned grassland vegetation. In the latter study area, five plots were placed in burned areas, five were positioned in unburned grasslands, and the five remaining plots were located in an area that contained a mix of burned and unburned patches of grassland vegetation.

Sampling Design - All of the plots in the Bouteloua gracilis-dominated site were 4 m x 16 m in dimension. Of the 25 plots where B. eriopoda was more abundant, nine were 4 m x 16 m, and 16 were 4 m x 25 m. Regardless of site, all plots were oriented such that the long axis of each was parallel to a topographic gradient that ran in an east-west direction.

We established three 1.5 m x 1.5 m quadrats at the two corners and midpoint along the south side of each plot. Each quadrat was divided into four square cells of equal area. Within a southeastern cell (northeastern cell if a shrub was present in a southeastern cell), we selected three grass clumps and three interspace areas between plants. At the center of each plant and interspace, one sample was removed at each of two depths (0 - 2.5 cm and 2.5 - 10cm). The three "grass" samples at each depth were pooled to create one composite sample; similarly, the three interspace samples at each depth were also pooled into one sample (4 composite samples in total).

Field Methods - For the 0 - 2.5 cm samples, a 2.5-cm corer (6.7 cm diameter) was driven into the ground with a mallet. Over grass clumps, the corer was inserted such that the average original soil surface was flush with the top of the corer, generally at about one-half the height of the tussock. A sharpened trowel was hammered beneath the corer to cut the roots, and the core was removed. Any soil which fell onto the new surface was scraped away.

For the 2.5 - 10 cm samples, a 25-cm corer (4.5 cm diameter) was driven to a depth of 7.5 cm below the surface created by the previous sample. The corer was then twisted and gently pulled up. The average point of break for any individual core was estimated to be within 1 - 2 mm for at least 90% of the cores.  The soil was resampled if this error exceeded 3 mm.

Samples were placed into pre-labeled paper lunch bags and transferred to a cooler (without ice). They were transported to a drying shed with ambient temperature and humidity and placed on shelves within 36 hours.

Laboratory Procedures - Soil moisture was determined by the gravimetric method (Gardner 1986). Soil texture was determined by the hydrometer method (Sheldrick and Wang 1993).

Gardner, W. H. 1986. Water content. Pages 493-544 in A. Kluite (editor), Methods of soil analysis, Part 1. Physical and minerological methods, agronomy monograph no. 9, 2nd edition. American Society of Agronomy and Soil Science Society of America, Madison, WI.

Sheldrick, B.H. and C. Wang. 1993. Particle size distribution. Pages 499-557 in M.R. Carter (editor), Soil sampling and methods of analysis. Canadian Society of Soil Science, Lewis Publishers, Ann Arbor, MI.

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